Genome analysis of Phytophthora cactorum strains associated with crown- and leather-rot in strawberry

Phytophthora cactorum has two distinct pathotypes that cause crown rot and leather in strawberry ( Fragaria × ananassa ). Strains of the pathotype can infect both rhizome (crown) fruit tissues, while strains only fruits strawberry. The genome a highly virulent strain, low three were sequenced using PacBio high fidelity (HiFi) long read sequencing. reads de novo assembled to 66.4–67.6 megabases genomes 178–204 contigs, with N50 values ranging from 892 1,036 kilobases. total number predicted complete genes five P. ranged 17,286 17,398. Orthology analysis identified core secretome 8,238 genes. Comparative genomic revealed differences composition potential virulence effectors, such as putative RxLR Crinklers, between pathotypes. Insertions, deletions, amino acid substitutions detected encoding elicitors beta elicitin cellulose-binding domain proteins compared suggesting mechanism for strain escape host recognition during compatible interaction results presented here highlight several effectors may facilitate tissue-specific colonization